The most important icons are of: Elijah (12th cent.) in the severe Comnenian style; St Nicholas (12th cent.) on a silver ground and surrounded by ten scenes from his life; Christ Pantocrator (14th cent.); St Kosmas and St Damian (14th cent.); the Panayia Glykofiloussa and the Deposition from the Cross (late 14th cent.); the Man of Sorrows (15th cent.); an altar door (15th cent.) bearing a depiction of the Annunciation and busts of David and Solomon at the top; the Annunciation (16th cent.); the Pantocrator (16th cent.) painted by a well-known icon-painter named Ioannis Permeniotis; the Panayia Hodegetria (16th cent.); and the Dormition of St Nicholas (16th cent.). In the semicircular part of the exhibition space are displayed three outstanding works by Kastorian ateliers: an icon of St Paraskevi carrying her own head, and two altar doors with a representation of the Annunciation.
Until 1998, the museum ran an educational programme for ten- to seventeen-year-olds titled In the World of Byzantine Icons, and one of its aims for 2000 is to resume the programme. It involved a guided tour of the museum and a detailed account of the stages of the making of an icon, accompanied by related activities and games.