A number of finds from ancient Amphipolis are displayed on the ground floor: a marble bust of a woman (4th cent. bc), a marble grave stela of an ephebe (5th cent. bc), a large gold finger ring and a gold olive wreath that were found in Macedonian Tomb 1 (300 bc), a headless marble statue of a woman wearing a peplos (1st cent. bc), and a portrait bust of the Roman empress Agrippina. In the next room are architectural members from the sanctuary of the goddess Parthenos in ancient Neapolis, together with a large collection of pots and figurines of the Archaic period from various workshops (Corinth, Cyclades, Chios)
On the first floor are antiquities from Galepsos, Oisyme, ancient Topeiros, Abdera, Mesembria, and ancient Tragilos. They include black-figure wares, clay figurines, clay sarcophagi, a painted cist grave, metal pots, and coins of the Macedonian kings. Some very important exhibits are a Cycladic amphora (7th cent. bc) and a red-figure hydria (4th cent. bc), which were found serving as cinerary urns in a cemetery at Oisyme, and a poros sarcophagus with a decorated interior from a sepulchral monument at ancient Tragilos (300 bc).
In the courtyard and the atrium there are grave and votive stelai with reliefs and inscriptions, and architectural members from various parts of Eastern Macedonia, dating to the Roman period.